Huarte Muniesa M P, Maluenda Colomer M D, Civeira Murillo M P, Medarde Agustín A, Prieto Valtueña J M
Centro de Investigaciones Biomédicas, Facultad de Medicina, Universidad de Navarra, Pamplona.
Med Clin (Barc). 1994 Nov 12;103(16):601-5.
To determine the incidence of postransfusion hepatitis (PTH) and hepatitis C virus (HCV) infection in blood recipients in Navarra and to evaluate the impact of excluding anti-HCV positive donors on the incidence of posttransfusion HCV infection.
248 transfusion recipients were included, 150 in a first period before anti-HCV(+) donors exclusion, and 98 in a second period after their exclusion. A serum sample from each patient was collected, bimonthly during the first month and monthly for six months, and another serum sample, twelve months after transfusion. ALT and anti-HCV were tested in each sample with ELISA and RIBA 2nd generation. HCV-RNA was determined with a nested polymerase chain reaction assay.
PTH incidence in 1989 was of 2.9%. This incidence decreased to 1.1% after anti-HCV ELISA 1st generation positive donors exclusion. At present, this incidence, with anti-HCV ELISA 2nd generation positive donors exclusion, is 0.8%. 80% of PTH were caused by HCV. 50% of HCV acute infection, confirmed by positive PCR, had normal ALT.
In Navarra, HPT incidence is below 1% after anti-HCV(+) donors exclusion. There are subclinics forms of HCV acute postransfusional infection with normal ALT.
确定纳瓦拉地区输血受者中输血后肝炎（PTH）和丙型肝炎病毒（HCV）感染的发生率，并评估排除抗-HCV阳性献血者对输血后HCV感染发生率的影响。
纳入248名输血受者，其中150名在排除抗-HCV（+）献血者之前的第一个时期，98名在排除之后的第二个时期。在第一个月每两个月采集一次每位患者的血清样本，持续六个月，每月采集一次，输血后十二个月再采集一份血清样本。用ELISA和第二代重组免疫印迹试验（RIBA）检测每个样本中的丙氨酸转氨酶（ALT）和抗-HCV。用巢式聚合酶链反应测定法测定HCV-RNA。
1989年PTH的发生率为2.9%。在排除第一代抗-HCV ELISA阳性献血者后，该发生率降至1.1%。目前，在排除第二代抗-HCV ELISA阳性献血者后，该发生率为0.8%。80%的PTH由HCV引起。经PCR证实的HCV急性感染中有50%的ALT正常。
在纳瓦拉地区，排除抗-HCV（+）献血者后HPT发生率低于1%。存在ALT正常的HCV输血后急性感染亚临床形式。
