Anthony F W, Wheeler T, Elcock C L, Pickett M, Thomas E J
Department of Obstetrics and Gynaecology, University of Southampton, Princess Anne Hospital, UK.
Placenta. 1994 Jul;15(5):557-61. doi: 10.1016/s0143-4004(05)80424-2.
Reverse transcription and the polymerase chain reaction (PCR) were used to detect vascular endothelial growth factor (VEGF) mRNA in human placental tissue and cultured placental fibroblasts obtained during the first trimester of pregnancy. The primers for VEGF corresponded to areas in exon 4 and exon 8 of the VEGF gene. After one round of PCR three products, equivalent to VEGF121, VEGF165 and VEGF189, were detected within placental tissue and cultured placental fibroblasts. A further round of PCR revealed the presence of two more products equivalent to VEGF206 and VEGF145. Thus, in addition to the production of readily secreted forms of VEGF (VEGF121 and VEGF165), the placenta produces several transcripts expected to increase the growth factor pool of the extracellular matrix.
采用逆转录和聚合酶链反应（PCR）检测孕早期人胎盘组织及培养的胎盘成纤维细胞中血管内皮生长因子（VEGF）mRNA。VEGF引物对应于VEGF基因外显子4和外显子8区域。一轮PCR后，在胎盘组织和培养的胎盘成纤维细胞中检测到三种产物，分别相当于VEGF121、VEGF165和VEGF189。进一步一轮PCR显示还存在另外两种产物，相当于VEGF206和VEGF145。因此，除了产生易于分泌形式的VEGF（VEGF121和VEGF165）外，胎盘还产生几种预期会增加细胞外基质生长因子库的转录本。
