Huovinen R, Collan Y
Laboratory of Electron Microscopy, University of Turku, Finland.
Tumour Biol. 1994;15(6):345-53.
The antiestrogen toremifene treatment results in regression or stabilization of dimethylbenz(a)anthracene(DMBA)-induced rat mammary tumors by reducing the mitotic activity and possibly by modifying the abundant spontaneous apoptosis seen in these tumors. A comparative study on the modes of cell loss was performed in untreated DMBA tumors and in tumors treated with toremifene and ovariectomy. Ovariectomy results in massive apoptotic cell death throughout the tumors with apoptotic cells exfoliating into the glandular lumina. Apoptosis accounts for most of the cell death also in the untreated tumors. The distinctly different morphology of the apoptotic cells in the untreated tumors (condensed apoptosis) and tumors treated with ovariectomy (blebbing apoptosis) may reflect the different type or schedule of the apoptotic process. Both morphologies of apoptotic cells were seen in the toremifene-treated tumors. Blebbing apoptosis is not easily examined in paraffin-embedded material but is best detectable in plastic sections and by electron microscopy.
抗雌激素药物托瑞米芬治疗可使二甲基苯并(a)蒽(DMBA)诱导的大鼠乳腺肿瘤消退或稳定，其机制是降低有丝分裂活性，并可能通过改变这些肿瘤中大量存在的自发凋亡来实现。对未治疗的DMBA肿瘤以及接受托瑞米芬和卵巢切除术治疗的肿瘤的细胞丢失模式进行了一项比较研究。卵巢切除术导致整个肿瘤出现大量凋亡性细胞死亡，凋亡细胞脱落至腺腔内。在未治疗的肿瘤中，凋亡也是细胞死亡的主要原因。未治疗肿瘤中凋亡细胞的形态（浓缩性凋亡）与接受卵巢切除术治疗的肿瘤中凋亡细胞的形态（泡状凋亡）明显不同，这可能反映了凋亡过程的不同类型或进程。在接受托瑞米芬治疗的肿瘤中可观察到这两种凋亡细胞形态。泡状凋亡在石蜡包埋材料中不易检测，但在塑料切片和电子显微镜下最易检测到。
