Huovinen R, Kellokumpu-Lehtinen P L, Collan Y
Laboratory of Electron Microscopy, University of Turku, Finland.
Int J Exp Pathol. 1994 Aug;75(4):257-63.
Evaluation of the treatment response to hormonal therapy in experimental mammary carcinoma models usually consists of recording the changes in tumour volume or in number of tumours. We performed quantitative histology on dimethylbenz[a]anthracene(DMBA) induced rat mammary carcinoma treated with the antioestrogen toremifene. The inhibition of growth of the treated regressing tumours was not only associated with inhibition of mitosis and reduction of the neoplastic epithelium, but toremifene seemed to reduce all main tissue components. The percentual area fractions of epithelium and stroma were equal in the untreated and the treated tumours. The estimated absolute volumes of epithelium, stroma and glandular luminae as well as the mitotic index were significantly reduced during toremifene treatment in those tumours which were responding by regression to the treatment. The findings indicate growth inhibiting effect on both epithelium and stroma or on interactions between epithelium and stroma. Adding quantitative histology to the gross response analysis in experimental animal models is feasible and may elucidate the mechanisms of action of a novel hormonal treatment.
在实验性乳腺癌模型中，对激素治疗反应的评估通常包括记录肿瘤体积或肿瘤数量的变化。我们对用抗雌激素药物托瑞米芬治疗的二甲基苯并[a]蒽（DMBA）诱导的大鼠乳腺癌进行了定量组织学研究。治疗后肿瘤消退生长的抑制不仅与有丝分裂的抑制和肿瘤上皮的减少有关，而且托瑞米芬似乎减少了所有主要组织成分。未治疗和治疗的肿瘤中上皮和基质的百分比面积分数相等。在那些对治疗有消退反应的肿瘤中，托瑞米芬治疗期间上皮、基质和腺腔的估计绝对体积以及有丝分裂指数显著降低。这些发现表明对上皮和基质或上皮与基质之间的相互作用有生长抑制作用。在实验动物模型的总体反应分析中增加定量组织学是可行的，并且可能阐明一种新型激素治疗的作用机制。
