Soulen M C, Sokol M C, Sullivan K L
Department of Radiology, Hospital of the University of Pennsylvania, Philadelphia 19104.
J Vasc Interv Radiol. 1994 Sep-Oct;5(5):765-9. doi: 10.1016/s1051-0443(94)71598-0.
To find a more effective chemical regimen for transcatheter ablation of the gallbladder in an in vitro model.
Sectioned and whole pig gallbladders were exposed in vitro to 12 different chemical solutions at varying conditions of exposure time, pH, and temperature.
In the in vitro studies, 0.1 N and 1.0 N solutions of sodium hydroxide in water or ethanol and 3% hydrogen peroxide were the most effective sclerosant agents. Ethanol and hydrochloric acid failed to completely eliminate the epithelium from the gallbladder sections. Increasing exposure time from 10 to 20 minutes or increasing the temperature of the solutions from 37 degrees C to 50 degrees C did not alter these results. Sequential 15-minute exposures to 0.1 N sodium hydroxide in ethanol followed by peroxide completely eliminated the epithelium from whole gallbladders in vitro.
Alkaline solutions and hydrogen peroxide are more effective than ethanol alone, acids, or detergents in eliminating gallbladder epithelium in this model. Further evaluation of these agents in vivo is merited.
在体外模型中寻找一种更有效的经导管胆囊消融化学方案。
将切片的和完整的猪胆囊在体外暴露于12种不同的化学溶液中，暴露时间、pH值和温度条件各不相同。
在体外研究中，水中或乙醇中的0.1 N和1.0 N氢氧化钠溶液以及3%过氧化氢是最有效的硬化剂。乙醇和盐酸未能完全去除胆囊切片上的上皮组织。将暴露时间从10分钟增加到20分钟或溶液温度从37摄氏度提高到50摄氏度并没有改变这些结果。在体外，先将完整胆囊依次暴露于乙醇中的0.1 N氢氧化钠15分钟，然后再暴露于过氧化氢15分钟，可完全去除上皮组织。
在该模型中，碱性溶液和过氧化氢在去除胆囊上皮组织方面比单独使用乙醇、酸或洗涤剂更有效。值得对这些试剂进行进一步的体内评估。
