Seyoum G, Persaud T V
Department of Anatomy, University of Manitoba, Winnipeg, Canada.
Exp Toxicol Pathol. 1994 Aug;46(3):177-81. doi: 10.1016/S0940-2993(11)80077-1.
S-adenosyl methionine (SAM) is a universal methyl donor for biological systems. Chronic consumption of ethanol results in depletion of available SAM and reduces its biosynthesis in the transmethylation pathway. Administration of excess SAM may reduce the embryopathic effects of ethanol. The in-vitro effects of SAM on ethanol embryopathy was investigated by culturing 9.5 day old whole rat embryos for 48 hours in ethanol alone (Group II), 0.05 mM SAM (Group III), ethanol + 0.05 mM SAM (Group IV), ethanol + 0.1 mM SAM (Group V), ethanol + 1 mM SAM (Group VI), and in ethanol + 3 mM SAM (Group VII). In Group VII embryos, cardiovascular, nervous, auditory, visual, craniofacial and musculoskeletal systems were retarded in development; crown-rump length, yolk-sac diameter, as well as morphological scores, were reduced compared to those in embryos treated with ethanol alone (Group II). There were, however, significant differences between Group II and Group IV embryos with respect to crown-rump length, yolk sac diameter and somite number. The mean crown-rump length, yolk sac diameter and somite number in Group II were 2.3 +/- 0.2, 2.8 +/- 0.3 and 22.4 +/- 3.5 respectively, compared to 2.6 +/- 0.2, 3.1 +/- 0.2 and 25.3 +/- 3.1 in Group IV. These results suggest that simultaneous administration of S-adenosyl methionine and ethanol may protect against the embryopathic effects of ethanol.
S-腺苷甲硫氨酸（SAM）是生物系统中一种通用的甲基供体。长期摄入乙醇会导致可用SAM耗竭，并减少其在转甲基途径中的生物合成。给予过量的SAM可能会降低乙醇的胚胎病效应。通过将9.5天大的全胚大鼠胚胎分别在单独的乙醇（第二组）、0.05 mM SAM（第三组）、乙醇 + 0.05 mM SAM（第四组）、乙醇 + 0.1 mM SAM（第五组）、乙醇 + 1 mM SAM（第六组）以及乙醇 + 3 mM SAM（第七组）中培养48小时，研究了SAM对乙醇胚胎病的体外影响。在第七组胚胎中，心血管、神经、听觉、视觉、颅面和肌肉骨骼系统的发育受到阻碍；与单独用乙醇处理的胚胎（第二组）相比，顶臀长度、卵黄囊直径以及形态学评分均降低。然而，第二组和第四组胚胎在顶臀长度、卵黄囊直径和体节数量方面存在显著差异。第二组的平均顶臀长度、卵黄囊直径和体节数量分别为2.3±0.2、2.8±0.3和22.4±3.5，而第四组分别为2.6±0.2、3.1±0.2和25.3±3.1。这些结果表明，同时给予S-腺苷甲硫氨酸和乙醇可能会预防乙醇的胚胎病效应。
