Seyoum G, Persaud T V
Department of Anatomy, University of Manitoba, Winnipeg, Canada.
Exp Toxicol Pathol. 1997 Aug;49(3-4):267-71. doi: 10.1016/S0940-2993(97)80028-0.
Organic zinc salts and thiols, administered simultaneously, protect mice synergistically against ethanol toxicity. Moreover, chronic ethanol consumption could affect the bioavailability of zinc and amino acids such as methionine. This could result in impaired embryonic growth and development. The influence of zinc and methionine on ethanol-induced embryopathy was investigated by simultaneous administration of ethanol, zinc and methionine to pregnant rats from gestational day 6 through 12. Ethanol was given in the form of a liquid diet; zinc administered i.p., and methionine was given by gavage. The ethanol group received the liquid ethanol diet; the ethanol + zinc and methionine group received the ethanol diet, zinc and methionine; and the pair-fed control group was given an isocaloric control diet. On day 12 of gestation, embryos of ethanol alone treated rats revealed a significantly reduced embryonic protein content, number of somites, crown-rump length, and lower morphological score (embryological differentiation) compared to the pair-fed control embryos. However, embryonic growth and developmental parameters in the ethanol, zinc and methionine treated group were not significantly different from those exposed to ethanol alone.
有机锌盐和硫醇同时给药时，可协同保护小鼠免受乙醇毒性影响。此外，长期摄入乙醇会影响锌和蛋氨酸等氨基酸的生物利用度。这可能导致胚胎生长发育受损。通过从妊娠第6天至12天对怀孕大鼠同时给予乙醇、锌和蛋氨酸，研究了锌和蛋氨酸对乙醇诱导的胚胎病的影响。乙醇以液体饲料的形式给予；锌腹腔注射给药，蛋氨酸通过灌胃给予。乙醇组接受液体乙醇饲料；乙醇+锌和蛋氨酸组接受乙醇饲料、锌和蛋氨酸；配对喂养对照组给予等热量对照饲料。在妊娠第12天，与配对喂养对照胚胎相比，仅用乙醇处理的大鼠胚胎显示胚胎蛋白含量、体节数、顶臀长度显著降低，形态学评分（胚胎分化）更低。然而，乙醇、锌和蛋氨酸处理组的胚胎生长和发育参数与仅暴露于乙醇的组无显著差异。
