从人肾中克隆推定的钙敏感受体cDNA的分子克隆
Molecular cloning of a putative Ca(2+)-sensing receptor cDNA from human kidney.
作者信息
Aida K, Koishi S, Tawata M, Onaya T
机构信息
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
出版信息
Biochem Biophys Res Commun. 1995 Sep 14;214(2):524-9. doi: 10.1006/bbrc.1995.2318.
A cDNA that encodes a putative Ca(2+)-sensing receptor (HuKCaSR) was cloned from human kidney with the use of the polymerase chain reaction. The predicted HuKCaSR protein consists of 1078 amino acids and shares 93.1 and 93.8% overall identity with the bovine parathyroid Ca(2+)-sensing receptor (BoPCaR1) and rat kidney Ca(2+)-sensing receptor (RaKCaR), respectively, with least similarity in the carboxyl-terminal regions. The HuKCaSR gene was mapped by fluorescence in situ hybridization to chromosome 3q21, at which region the gene responsible for familial hypocalciuric hypercalcemia has previously been localized by genetic linkage analyses. RNA blot analysis revealed HuKCaSR mRNA in human kidney, but not in brain, lung, liver, heart, skeletal muscle, or placenta.
利用聚合酶链反应从人肾脏中克隆出一个编码假定的钙（Ca²⁺）敏感受体（HuKCaSR）的互补脱氧核糖核酸（cDNA）。预测的HuKCaSR蛋白由1078个氨基酸组成，与牛甲状旁腺钙（Ca²⁺）敏感受体（BoPCaR1）和大鼠肾脏钙（Ca²⁺）敏感受体（RaKCaR）的总体一致性分别为93.1%和93.8%，在羧基末端区域的相似性最低。通过荧光原位杂交将HuKCaSR基因定位到3号染色体q21区域，此前通过遗传连锁分析已将导致家族性低钙血症性高钙血症的基因定位在该区域。RNA印迹分析显示HuKCaSR信使核糖核酸（mRNA）存在于人肾脏中，但在脑。肺、肝、心脏、骨骼肌或胎盘中不存在。
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