Stalheim O H, Proctor S J, Gallagher J E
Infect Immun. 1976 Mar;13(3):915-25. doi: 10.1128/iai.13.3.915-925.1976.
Ureaplasmas isolated from the human genital tract and from the genital and respiratory tracts of cattle were grown in association with organ cultures of bovine oviduct (uterine tube). All strains of unreaplasmas multiplied in organ cultures, stopped ciliary activity, and caused histological lesions. Most strains grew well, and 10(8) to 10(9) color-changing units were determined 18 to 144 h after inoculation. Twenty-four to 144 h after inoculation with unreaplasmas, ciliostasis was complete. Ciliostasis was also caused by additions of nonviable cultures at pH 8.8 (or adjusted to 7.4) or washed disrupted cells (100 mug of protein/ml); it occurred in 48 to 96 h. The cilia-stopping effect of nonviable cultures was diminished by heating (56 C for 30 min) and was abolished by boiling. When added to fresh medium in amounts exceeding 25%, nonviable unreaplasmal cultures completely inhibited ureaplasmal growth. By light, scanning, and transmission electron microscopy, cilia-stopping effect was correlated with collapse and sloughing of the cilia (the initial lesion was "bent" cilia), with bulging and vacuolization of secretory and ciliated cells, and finally with disorganization of the epithelium, necrosis, and desquamation.
从人类生殖道以及牛的生殖道和呼吸道分离出的脲原体，与牛输卵管（子宫管）的器官培养物共同培养。所有脲原体菌株在器官培养物中繁殖，停止纤毛活动，并引起组织学病变。大多数菌株生长良好，接种后18至144小时可检测到10⁸至10⁹个颜色改变单位。接种脲原体后24至144小时，纤毛静止完全。在pH 8.8（或调至7.4）的无活力培养物或洗涤后的破碎细胞（100μg蛋白质/毫升）添加时也会引起纤毛静止；这发生在48至96小时。无活力培养物的纤毛停止作用通过加热（56℃ 30分钟）而减弱，通过煮沸而消除。当以超过25%的量添加到新鲜培养基中时，无活力的脲原体培养物完全抑制脲原体生长。通过光学、扫描和透射电子显微镜观察，纤毛停止作用与纤毛的塌陷和脱落（初始病变为“弯曲”纤毛）、分泌细胞和纤毛细胞的肿胀及空泡化相关，最终与上皮组织紊乱、坏死和脱屑相关。
