Kodera Y, Isobe K, Yamauchi M, Kondo K, Akiyama S, Ito K, Nakashima I, Takagi H
Department of Surgery II, Nagoya University School of Medicine, Japan.
Cancer Chemother Pharmacol. 1994;34(3):203-8. doi: 10.1007/BF00685078.
Glutathione-S-transferase (GST) in one of several factors that are proposed to affect tumor sensitivity to anticancer drugs, including cisplatin (CDDP). Attempts are made herein to evaluate the significance of the enzymes in resistance to CDDP in clinical samples of gastric cancer. A total of 22 gastric cancer specimens, 16 of which were obtained with matching normal mucosae, underwent immunoblotting with polyclonal antibodies against GST-alpha and GST-pi. At the same time, the chemosensitivity of 15 gastric cancer specimens to CDDP was evaluated by the succinic dehydrogenase inhibition (SDI) test. The expression of GST-pi was detected in all the specimens, and its content in the neoplasms exhibited a significant positive correlation with that in the matched normal mucosae. The expression of GST-alpha was detected in 18 of 22 cancer specimens (82%), but its content in the neoplasms did not correlate with that in the matched mucosae. A comparison of the drug-sensitivity findings with the results of immunoblotting revealed a weak but interesting correlation between the protein levels of GST-alpha and CDDP resistance. The cellular content of GST-alpha correlated weakly with CDDP resistance in gastric cancer, and its quantification could contribute to prediction of the clinical effects of CDDP in patients with gastric cancer.
谷胱甘肽 - S - 转移酶（GST）是被认为会影响肿瘤对包括顺铂（CDDP）在内的抗癌药物敏感性的多种因素之一。本文旨在评估该酶在胃癌临床样本中对顺铂耐药性的意义。总共22个胃癌标本，其中16个与匹配的正常黏膜一起获取，用抗GST - α和GST - π的多克隆抗体进行免疫印迹分析。同时，通过琥珀酸脱氢酶抑制（SDI）试验评估15个胃癌标本对顺铂的化学敏感性。在所有标本中均检测到GST - π的表达，其在肿瘤中的含量与匹配的正常黏膜中的含量呈显著正相关。在22个癌标本中的18个（82%）检测到GST - α的表达，但其在肿瘤中的含量与匹配黏膜中的含量无相关性。将药物敏感性结果与免疫印迹结果进行比较，发现GST - α蛋白水平与顺铂耐药性之间存在微弱但有趣的相关性。GST - α的细胞含量与胃癌中的顺铂耐药性呈弱相关，其定量分析有助于预测顺铂对胃癌患者的临床疗效。
