Smith H E, Jacobs R M, Mallard B
Department of Pathology, University of Guelph, Ontario, Canada.
Comp Immunol Microbiol Infect Dis. 1994 Feb;17(1):29-39. doi: 10.1016/0147-9571(94)90004-3.
Six sheep were transfused intraperitoneally with whole blood from two sheep chronically infected with the bovine immunodeficiency-like virus (BIV). Five sheep were transfused intraperitoneally (i.p.) with normal ovine whole blood and served as controls. Five of six BIV-inoculated sheep seroconverted; four were transiently seropositive while one remained seropositive for the duration of the experiment. Tests for nonspecific lymphocyte reactivity to mitogens were performed monthly for one year. At approximately 10 months postinoculation, all sheep were immunized with chicken ovalbumin, canine red blood cells, and tuberculin. There were no significant associations between BIV exposure and deficits in antibody production to chicken ovalbumin and canine red blood cells; nonspecific lymphoproliferative responses to concanavalin-A, lipopolysaccharide, and pokeweed mitogen; specific lymphoproliferative responses to ovalbumin and tuberculin purified protein derivative; or cutaneous delayed type hypersensitivity to tuberculin purified protein derivative. Exposure to BIV did not alter the humoral or cell mediated immune responses of sheep in the first year of exposure.
给六只绵羊腹腔内输注来自两只长期感染牛免疫缺陷样病毒（BIV）绵羊的全血。五只绵羊腹腔内（i.p.）输注正常绵羊全血并作为对照。六只接种BIV的绵羊中有五只发生血清转化；四只呈短暂血清阳性，而一只在实验期间一直保持血清阳性。对有丝分裂原的非特异性淋巴细胞反应性测试持续进行了一年，每月进行一次。在接种后约10个月，所有绵羊均用鸡卵白蛋白、犬红细胞和结核菌素进行免疫。BIV暴露与针对鸡卵白蛋白和犬红细胞的抗体产生缺陷、对刀豆蛋白A、脂多糖和商陆有丝分裂原的非特异性淋巴细胞增殖反应、对卵白蛋白和结核菌素纯化蛋白衍生物的特异性淋巴细胞增殖反应或对结核菌素纯化蛋白衍生物的皮肤迟发型超敏反应之间均无显著关联。在暴露的第一年，接触BIV并未改变绵羊的体液免疫或细胞介导免疫反应。
