精子-免疫珠结合随着体外孵育而降低。
Sperm-immunobead binding decreases with in vitro incubation.
作者信息
Gould J E, Brazil C K, Overstreet J W
机构信息
University of California, School of Medicine, Davis.
出版信息
Fertil Steril. 1994 Jul;62(1):167-71. doi: 10.1016/s0015-0282(16)56834-4.
OBJECTIVE
To characterize the stability of the sperm-immunobead association over time.
DESIGN
Prospective evaluation of sperm-immunobead binding, using direct and indirect assays.
SETTING
Male Infertility Clinic, University of California, Davis, Davis, California.
PATIENTS
Eleven men with sperm surface antibodies and 25 men with serum antisperm antibodies volunteered.
MAIN OUTCOME MEASURES
Repeated assessment of sperm-immunobead binding over time.
RESULTS
Serum immunoglobulin (Ig)G decreased a mean of 42.6% over 30 minutes, and serum IgA decreased a mean of 22.7% over 30 minutes. Semen-derived IgG binding fell a mean of 59.9% and semen-derived IgA fell a mean of 27.0% over 25 to 40 minutes.
CONCLUSIONS
Sperm-immunobead coincubation results in a decrease in the number of sperm bound to immunobeads.
目的
描述精子 - 免疫珠结合随时间的稳定性。
设计
采用直接和间接检测方法对精子 - 免疫珠结合进行前瞻性评估。
地点
加利福尼亚大学戴维斯分校男性不育诊所，加利福尼亚州戴维斯市。
患者
11名有精子表面抗体的男性和25名有血清抗精子抗体的男性自愿参与。
主要观察指标
随时间对精子 - 免疫珠结合进行重复评估。
结果
血清免疫球蛋白（Ig）G在30分钟内平均下降42.6%，血清IgA在30分钟内平均下降22.7%。精液来源的IgG结合在25至40分钟内平均下降59.9%，精液来源的IgA平均下降27.0%。
结论
精子 - 免疫珠共孵育导致与免疫珠结合的精子数量减少。
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