Gillett W R, James C, Jetha N, McComb P F
Department of Obstetrics and Gynaecology, University of Otago, Dunedin, New Zealand.
Hum Reprod. 1994 Mar;9(3):497-500. doi: 10.1093/oxfordjournals.humrep.a138534.
To assess the effect of the removal of ovarian surface epithelium on repair, a standard injury was induced in the ovaries of 10 rabbits. In one ovary the surface cells were denuded, and in the other they were left intact. The effect on adhesion formation was assessed at 12 days. Adhesions were assessed by visual inspection at laparotomy and histological examination of adhesion formation, including a stereological assessment of scar volume. On visual assessment the overall adhesion scores for the denuded ovaries were greater than for the intact ovaries. Histology showed the adhesions were attached only to the site of injury. The Fallopian tube was adherent to 35 and 4% of the denuded and intact ovarian segments respectively (P = 0.003). The scar volumes for each side were similar. After 12 days there was only partial re-epithelialization on the denuded ovaries. Electron microscopy confirmed the slow healing, with much of the surface still covered by a fibrinous-like exudate. The findings of this small study lend further weight to the importance of the surface epithelium in the control of adhesion formation. Standard surgical procedures may generate adhesions by the inadvertent denuding of surface epithelium from adjacent healthy tissues, possibly by the loss of plasminogen activator activity that is found in the mesothelium of the peritoneum. This study highlights the importance of controlling for inadvertent cell loss whilst investigating methods for adhesion prevention.
为评估去除卵巢表面上皮对修复的影响，在10只兔子的卵巢上造成标准损伤。一侧卵巢的表面细胞被剥除，另一侧保持完整。在第12天评估对粘连形成的影响。通过剖腹手术时的目视检查以及粘连形成的组织学检查（包括对瘢痕体积的体视学评估）来评估粘连情况。目视评估显示，剥除表面上皮的卵巢的总体粘连评分高于完整卵巢。组织学检查表明，粘连仅附着于损伤部位。输卵管分别与35%的剥除表面上皮的卵巢节段和4%的完整卵巢节段粘连（P = 0.003）。两侧的瘢痕体积相似。12天后，剥除表面上皮的卵巢仅出现部分再上皮化。电子显微镜证实愈合缓慢，大部分表面仍被纤维蛋白样渗出物覆盖。这项小型研究的结果进一步证明了表面上皮在控制粘连形成中的重要性。标准外科手术可能因不经意间剥除相邻健康组织的表面上皮而产生粘连，这可能是由于腹膜间皮中发现的纤溶酶原激活物活性丧失所致。这项研究突出了在研究预防粘连方法时控制不经意间细胞丢失的重要性。
