Stanford C M, Keller J C, Solursh M
Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City 52242.
J Dent Res. 1994 May;73(5):1061-71. doi: 10.1177/00220345940730050801.
Phenotypic responses of rat calvarial osteoblast-like cells (RCOB) were evaluated on commercially pure titanium (cpTi) surfaces when cultured at high density (5100 cells/mm2). These surfaces were prepared to three different clinically relevant surface preparations (1-micron, 600-grit, and 50-microns-grit sand-blast), followed by sterilization with either ultraviolet light, ethylene oxide, argon plasma-cleaning, or routine clinical autoclaving. Osteocalcin and alkaline phosphatase, but not collagen expression, were significantly affected by surface roughness when these surfaces were altered by argon plasma-cleaning. In general, plasma-cleaned cpTi surfaces demonstrated an inverse relationship between surface roughness and phenotypic markers for a bone-like response. On a per-cell basis, levels of the bone-specific protein, osteocalcin, and the enzymatic activity of alkaline phosphatase were highest on the smooth 1-micron polished surface and lowest on the roughest surfaces for the plasma-cleaned cpTi. Detectable bone cell expression can be altered by clinically relevant surfaces prepared by standard dental implant preparation techniques.
当以高密度（5100个细胞/mm²）培养时，对商业纯钛（cpTi）表面上的大鼠颅骨成骨样细胞（RCOB）的表型反应进行了评估。这些表面被制备成三种不同的具有临床相关性的表面处理（1微米、600目和50微米喷砂），随后分别用紫外线、环氧乙烷、氩等离子体清洗或常规临床高压灭菌进行消毒。当通过氩等离子体清洗改变这些表面时，骨钙素和碱性磷酸酶（而非胶原蛋白表达）受到表面粗糙度的显著影响。一般来说，对于类骨反应，经等离子体清洗的cpTi表面显示出表面粗糙度与表型标志物之间呈反比关系。在单个细胞基础上，对于经等离子体清洗的cpTi，骨特异性蛋白骨钙素的水平以及碱性磷酸酶的酶活性在光滑的1微米抛光表面上最高，而在最粗糙的表面上最低。通过标准牙种植体制备技术制备的具有临床相关性的表面可以改变可检测到的骨细胞表达。
