Polymer Centre, Faculty of Technology, Tomas Bata University in Zlín, T.G.M Sq. 275, Zlín, Czech Republic.
Molecules. 2010 Apr 20;15(4):2845-56. doi: 10.3390/molecules15042845.
Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
采用等离子体化学反应器对类人胶原蛋白膜进行氩气等离子体处理，以改变其表面性质。为了评估处理效果，使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、扫描电子显微镜（SEM）成像和 X 射线光电子能谱（XPS）技术对未处理和处理过的样品进行了表征。通过培养人永生化角质形成细胞（HaCaT）进行细胞生长，并通过 MTT 测定法测量增殖情况。结果表明，氩气等离子体处理显著增强了细胞增殖程度，这归因于等离子体处理在诱导表面含氧基团以及增加表面粗糙度方面的有利作用。这可以被认为是一种有前途的组织再生方法。
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