Luo S R, Li T, Yang J S
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Yao Xue Xue Bao. 1993;28(9):695-8.
Gelsemium elegans Benth is a kind of traditional Chinese medicine, eight alkaloids have been isolated from this herb. In recent years, an HPLC method for the separation and determination of five of these alkaloids, ie. gelsemium A (G), koumine (F), kumantenidine (D), kumantenine (B) and kumantenmine (A) is described, dichroine being used as the internal standard. In this report a RP column of C18 and the mobile phase methanol--water--n-butylamine (78:22:0.1 V/V) were employed. The flow rate was 1.0 ml/min, the column temperature was 25 degrees C and the detection wavelength was 256 nm. The calibration curves showed good linearity over the range of 0.02-0.12 microgram, r = 0.9835-0.9977 and the recoveries were 95.01-99.70% for the five alkaloids. The method is simple, sensitive and reproducible and can be used for the quality control of Gelsemium preparations for clinical evaluation.
钩吻是一种传统中药，已从该草药中分离出八种生物碱。近年来，描述了一种高效液相色谱法用于分离和测定其中五种生物碱，即钩吻素甲（G）、钩吻碱（F）、钩吻定碱（D）、钩吻素子（B）和钩吻素寅（A），以二氢钩吻碱作为内标。在本报告中，使用了C18反相柱和流动相甲醇 - 水 - 正丁胺（78:22:0.1 V/V）。流速为1.0 ml/min，柱温为25℃，检测波长为256 nm。校准曲线在0.02 - 0.12微克范围内显示出良好的线性，r = 0.9835 - 0.9977，五种生物碱的回收率为95.01 - 99.70%。该方法简单、灵敏且可重现，可用于钩吻制剂临床评价的质量控制。
