灵芝多糖的免疫调节和抗肿瘤活性研究。
Studies on the immuno-modulating and anti-tumor activities of Ganoderma lucidum (Reishi) polysaccharides.
作者信息
Chen Hung-Sen, Tsai Yow-Fu, Lin Steven, Lin Chia-Ching, Khoo Kay-Hooi, Lin Chun-Hung, Wong Chi-Huey
机构信息
The Genomic Research Center, Academia Sinica, No. 128 Academia Road, Section 2, Nan-Kang, Taipei 11529, Taiwan.
出版信息
Bioorg Med Chem. 2004 Nov 1;12(21):5595-601. doi: 10.1016/j.bmc.2004.08.003.
We describe here the isolation of Reishi polysaccharides for the study of their effect on cytokine expression in mouse splenocytes. A fraction (F3) has been shown to activate the expression of IL-1, IL-6, IL-12, IFN-gamma, TNF-alpha, GM-CSF, G-CSF, and M-CSF, and from this three subfractions have been prepared where F3G1 activates IL-1, IL-12, TNF-alpha, and G-CSF, F3G2 activates all the cytokines as F3 does, and F3G3 activates only IL-1 and TNF-alpha. Together with previous studies, the mode of action on macrophages has been proposed where F3 binds to TLR4 receptor and activates extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 to induce IL-1 expression.
我们在此描述了从灵芝中分离多糖以研究其对小鼠脾细胞中细胞因子表达影响的过程。已证明其中一个组分（F3）可激活白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-12（IL-12）、干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、粒细胞集落刺激因子（G-CSF）和巨噬细胞集落刺激因子（M-CSF）的表达，并且由此制备了三个亚组分，其中F3G1激活IL-1、IL-12、TNF-α和G-CSF，F3G2与F3一样激活所有细胞因子，而F3G3仅激活IL-1和TNF-α。结合先前的研究，已提出其对巨噬细胞的作用模式，即F3与Toll样受体4（TLR4）结合并激活细胞外信号调节激酶（ERK）、c-Jun氨基末端激酶（JNK）和p38以诱导IL-1表达。
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