Arankalle V A, Favorov M O, Chadha M S, Phule D M, Banerjee K
National Institute of Virology, Pune, India.
Acta Virol. 1993 Dec;37(6):515-8.
Two rhesus monkeys (M. mullata) of approximately two years of age were inoculated intravenously with a 10% suspension of hepatitis E virus (HEV) positive stool from Kirghistan as evidenced by immuno-electron microscopy. Evidence of HEV infection was demonstrated by rise in serum alanine transaminase (ALT) levels and seroconversion of these monkeys to anti-HEV after 1-1/2 months post-inoculation as evidenced by immunoblot. One year after the primary inoculation, these monkeys were challenged with an Indian strain of HEV. No rise in serum ALT levels was noted during an observation period of 6 months. The same inoculum produced HE in two rhesus monkeys. The results showed that strains from India and Kirghistan were antigenically closely related and rhesus monkeys infected with one strain of virus were immune to another strain.
两只约两岁的恒河猴经静脉接种了来自吉尔吉斯斯坦的戊型肝炎病毒（HEV）阳性粪便的10%悬液，免疫电子显微镜证实了这一点。接种后1个半月，血清丙氨酸转氨酶（ALT）水平升高以及这些猴子通过免疫印迹法检测出抗HEV血清学转换，证明了HEV感染。初次接种一年后，这些猴子用一株印度株HEV进行攻击。在6个月的观察期内未观察到血清ALT水平升高。相同的接种物在另外两只恒河猴中引发了戊型肝炎。结果表明，来自印度和吉尔吉斯斯坦的毒株在抗原性上密切相关，感染一种病毒株的恒河猴对另一种病毒株具有免疫力。
