Arankalle V A, Goverdhan M K, Banerjee K
National Institute of Virology, Pune, India.
J Viral Hepat. 1994;1(2):125-9. doi: 10.1111/j.1365-2893.1994.tb00111.x.
To examine whether Indian monkeys are infected with hepatitis E virus (HEV) in nature, serum samples from wild rhesus (Macaca mullata), bonnet (M. radiata) and langur (Presbytes entellus) monkeys were screened for anti-HEV IgG antibodies in recombinant antigen-based ELISA assays. The positivity rates were 36.7%, 19.1% and 2% respectively. The protection of such antibodies against human HEV was studied in four rhesus monkeys. Of the two rhesus monkeys with anti-HEV titres of 100 and 1000 respectively which were inoculated with the KOL-91 strain of HEV, the former demonstrated a 10-fold rise in anti-HEV titres. Anti-HEV titre in the second rhesus monkey remained unchanged. Neither of the monkeys showed any rise in serum alanine transaminase (ALT) or presence of virus in the faeces, as tested by polymerase chain reaction (PCR). Two other rhesus monkeys with anti-HEV titres of 10,000 and 100 respectively were inoculated with the AKL-90 strain of HEV. Serum ALT levels and anti-HEV titres remained unchanged in the first monkey. Excretion of virus in faeces was not noted (PCR). The second monkey developed a typical HEV infection. HEV infection could be produced in anti-HEV negative control monkeys inoculated with both strains of HEV. These results show that either human or simian HEV, or a closely related agent, is circulating among Indian macaques. Titre-dependent protection of naturally occurring anti-HEV antibodies supports this view.
为检测印度猴子在自然环境中是否感染戊型肝炎病毒（HEV），采用基于重组抗原的酶联免疫吸附测定（ELISA）法，对野生恒河猴（猕猴）、圆帽猴和叶猴的血清样本进行抗HEV IgG抗体筛查。阳性率分别为36.7%、19.1%和2%。在4只恒河猴中研究了此类抗体对人HEV的保护作用。在分别接种HEV KOL - 91株的2只抗HEV滴度分别为100和1000的恒河猴中，前者抗HEV滴度升高了10倍。第二只恒河猴的抗HEV滴度未变。经聚合酶链反应（PCR）检测，两只猴子的血清丙氨酸转氨酶（ALT）均未升高，粪便中也未检测到病毒。另外两只抗HEV滴度分别为10000和100的恒河猴接种了HEV AKL - 90株。第一只猴子的血清ALT水平和抗HEV滴度未变。粪便中未检测到病毒排出（PCR）。第二只猴子发生了典型的HEV感染。接种两种HEV株的抗HEV阴性对照猴子均可发生HEV感染。这些结果表明，人源或猿源HEV或密切相关病原体正在印度猕猴中传播。天然存在的抗HEV抗体的滴度依赖性保护支持这一观点。
