Naito M, Imamura M, Kannagi R, Tobe T
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Jpn J Surg. 1990 Mar;20(2):170-9. doi: 10.1007/BF02470765.
The immunization of Balb/C mice with esophageal cell line KYSE-50 established from poorly-differentiated esophageal squamous cell carcinoma, resulted in obtaining the monoclonal antibody KYMN-28-5. This monoclonal antibody is of the IgM class and recognizes a carbohydrate antigen contained in glycoproteins with molecular weights of 53 and 56K, and in neutral glycolipids extracted from teratomas. Tissue staining revealed that this monoclonal antibody reacts strongly with malignant tumors but only weakly, or not at all, with normal tissues, apart from squamous epithelial tissue. KYMN-28-5 is thus a useful tumor marker which will improved the accuracy of serological diagnosing squamous cell carcinoma when combined with the measurement of SCC antigen.
用源自低分化食管鳞状细胞癌的食管细胞系KYSE - 50对Balb/C小鼠进行免疫，获得了单克隆抗体KYMN - 28 - 5。该单克隆抗体属于IgM类，可识别分子量为53和56K的糖蛋白中所含的一种碳水化合物抗原，以及从畸胎瘤中提取的中性糖脂中的该抗原。组织染色显示，除鳞状上皮组织外，该单克隆抗体与恶性肿瘤强烈反应，但与正常组织反应微弱或无反应。因此，KYMN - 28 - 5是一种有用的肿瘤标志物，与鳞状细胞癌抗原（SCC抗原）检测相结合时，将提高血清学诊断鳞状细胞癌的准确性。
