Takamizawa K, Iwamori M, Mutai M, Nagai Y
J Biol Chem. 1986 Apr 25;261(12):5625-30.
Bovine buttermilk was found to contain 0.92 mumol of lipid-bound sialic acid/g dry weight. On ganglioside mapping, at least seven gangliosides were detected, and the structures of the five major molecules were determined by degradation with exoglycosidases and methylation analysis. GM3, GD3, and GT3 were found to comprise 80% of the total gangliosides. The other two gangliosides had a new core structure with a branched oligosaccharide chain. One was a novel monosialoganglioside with a 2----6 linked sialic acid residue with the following structure: (Formula, see text) There were 41 nmol of this ganglioside/g of buttermilk (4.5% of total gangliosides). The other was a trisialogangliosides with the above new core structure.
发现牛乳清中每克干重含有0.92微摩尔脂质结合唾液酸。在神经节苷脂图谱分析中，检测到至少七种神经节苷脂，通过外切糖苷酶降解和甲基化分析确定了五种主要分子的结构。发现GM3、GD3和GT3占总神经节苷脂的80%。另外两种神经节苷脂具有带有分支寡糖链的新核心结构。一种是新型单唾液酸神经节苷脂，带有一个2----6连接的唾液酸残基，其结构如下：（分子式，见正文）每克乳清中有41纳摩尔这种神经节苷脂（占总神经节苷脂的4.5%）。另一种是具有上述新核心结构的三唾液酸神经节苷脂。
