[Effect of ethanol and phenobarbital on mutagenic properties of acid green pure V].

The frequency of micronuclei was evaluated in polychromatic erythrocytes of bone marrow in rats exposed to acid green pure V, ethanol and phenobarbital, and to joint action of all these substances. The experiment was carried out on 45 Wistar rats receiving acid green pure V in doses of 75 mg/kg b.w., 10% ethanol solution in drinking water for 90 days and phenobarbital. A significant rise was observed in the number of micronuclei in polychromatic erythrocytes in bone marrow of rats exposed to acid green pure V alone or in combination with phenobarbital or with ethanol. Ethanol and phenobarbital had no significant effect on the frequency of micronuclei as compared with control animals, they caused also no mutagenic changes and no increase in the genotoxic action of acid green pure V.