Moroo I, Yamada T, Makino H, Tooyama I, McGeer P L, McGeer E G, Hirayama K
Department of Neurology, Chiba University School of Medicine, Japan.
Acta Neuropathol. 1994;87(4):343-8. doi: 10.1007/BF00313602.
Immunohistochemistry using both a newly developed polyclonal, and a commercially available monoclonal, anti-insulin receptor antibody was done on the midbrain from cases of idiopathic Parkinson's disease (PD), Alzheimer's disease, amyotrophic lateral sclerosis, vascular parkinsonism and non-neurological controls. Both antibodies gave identical patterns of neuronal staining. The neurons of the oculomotor nucleus were immunopositive in all the brains. However, the neurons in the pars compacta of the substantia nigra, paranigral nucleus, parabrachial pigmental nucleus, tegmental pedunculopontine nucleus, supratrocheal nucleus, cuneiform nucleus, subcuneiform nucleus and lemniscus medialis, which were positive in other diseases and in non-neurological controls, were not stained by these antibodies in PD brains. These results suggest that, in PD, a dysfunction of the insulin/insulin receptor system may precede death of the dopaminergic neurons.
使用新开发的多克隆抗体和市售单克隆抗胰岛素受体抗体，对特发性帕金森病（PD）、阿尔茨海默病、肌萎缩侧索硬化症、血管性帕金森综合征病例以及非神经学对照的中脑进行免疫组织化学检测。两种抗体给出了相同的神经元染色模式。动眼神经核的神经元在所有大脑中均呈免疫阳性。然而，黑质致密部、黑质旁核、臂旁色素核、被盖脚桥核、滑车神经上核、楔形核、楔下核和内侧丘系中的神经元，在其他疾病和非神经学对照中呈阳性，但在PD大脑中未被这些抗体染色。这些结果表明，在PD中，胰岛素/胰岛素受体系统功能障碍可能先于多巴胺能神经元死亡。
