Van Dyk T K, Majarian W R, Konstantinov K B, Young R M, Dhurjati P S, LaRossa R A
DuPont Company, Wilmington, Delaware 19880.
Appl Environ Microbiol. 1994 May;60(5):1414-20. doi: 10.1128/aem.60.5.1414-1420.1994.
Heat shock gene expression is induced by a variety of environmental stresses, including the presence of many chemicals. To address the utility of this response for pollutant detection, two Escherichia coli heat shock promoters, dnaK and grpE, were fused to the lux genes of Vibrio fischeri. Metals, solvents, crop protection chemicals, and other organic molecules rapidly induced light production from E. coli strains containing these plasmid-borne fusions. Introduction of an outer membrane mutation, tolC, enhanced detection of a hydrophobic molecule, pentachlorophenol. The maximal response to pentachlorophenol in the tolC+ strain was at 38 ppm, while the maximal response in an otherwise isogenic tolC mutant was at 1.2 ppm. Stress responses were observed in both batch and chemostat cultures. It is suggested that biosensors constructed in this manner may have potential for environmental monitoring.
热休克基因表达可由多种环境压力诱导，包括许多化学物质的存在。为了探究这种反应在污染物检测中的效用，将两个大肠杆菌热休克启动子dnaK和grpE与费氏弧菌的lux基因融合。金属、溶剂、农作物保护化学品和其他有机分子能迅速诱导含有这些质粒携带融合体的大肠杆菌菌株发光。引入外膜突变tolC可增强对疏水分子五氯苯酚的检测。tolC+菌株对五氯苯酚的最大反应浓度为38 ppm，而在其他方面同基因的tolC突变体中的最大反应浓度为1.2 ppm。在分批培养和恒化器培养中均观察到应激反应。有人认为，以这种方式构建的生物传感器可能具有环境监测的潜力。
