Zolotova L I, Andrianov V V, Gorelova T V, Klitsunova N V, Reznik N L, Shuppe N G
Genetika. 1996 Nov;32(11):1528-35.
Data on the molecular arrangement of viruslike particles (VLPs) of yeast and Drosophila retrotransposons are presented. Two methods for identifying VLPs from specific retrotransposon families have been offered. The first method is based on VLPs fractionation by electrophoresis in agarose gel under strictly controlled conditions. VLPs of the Drosophila melanogaster retrotransposon families copia and gypsy and D. virilis retrotransposon Tv1 were identified by this method. The method based on heterologous induction of retrotransposons in cells of the mutant spt3 strain of Saccharomyces cerevisiae was used to identify VLPs of yeast retrotransposon Tyl and D. melanogaster gypsy retrotransposon.
本文展示了酵母和果蝇逆转录转座子的病毒样颗粒(VLP)的分子排列数据。文中提供了两种从特定逆转录转座子家族中鉴定VLP的方法。第一种方法是基于在严格控制的条件下,通过琼脂糖凝胶电泳对VLP进行分级分离。利用这种方法鉴定出了黑腹果蝇逆转录转座子家族copia和gypsy以及 virilis逆转录转座子Tv1的VLP。基于在酿酒酵母突变体spt3菌株细胞中逆转录转座子的异源诱导的方法,被用于鉴定酵母逆转录转座子Tyl和黑腹果蝇gypsy逆转录转座子的VLP。