[Results of cell separation with new single needle techniques in comparison with a current dual needle technique].

BACKGROUND

In plateletpheresis, new single-needle techniques have been introduced for the cell separators A 201 and AS 104. These techniques were compared with the well established CS 3000 Plus cell separator, which uses the continuous flow system.

MATERIAL AND METHODS

Donation time, ACD consumption, platelet yield, separation efficiency, erythrocyte and leukocyte contamination were studied in different protocols with the dual- and single-needle procedure.

RESULTS

Total amount of thrombocytes and separation efficacy were lowest with the A 201 device. The separation efficiency was best with the single-needle version of the AS 104 separator due to reprocession of the blood during the return cycle to the centrifuge. Leukocyte contamination was lowest with the standard procedure of the CS 3000 Plus device.

CONCLUSIONS

The single-needle technique of the AS 104 blood cell separator resulted in sufficient platelet collection. The purity of the platelet concentrates collected with the A 201 and AS 104 machine has to be improved.