Rao M, Liu F S, Dawsey S M, Yang K, Lipkin M, Li J Y, Taylor P R, Li B, Blot W J, Wang G Q
National Cancer Institute, Bethesda, Maryland 20892.
Cancer Epidemiol Biomarkers Prev. 1994 Apr-May;3(3):277-9.
Abnormalities of epithelial proliferation have been proposed as an early step in gastrointestinal carcinogenesis. To determine whether micronutrient supplementation may reduce squamous epithelial proliferation in the esophagus, we evaluated proliferation in subjects participating in a randomized nutrition intervention trial in Linxian, China, where esophageal cancer rates are among the highest in the world. After 30 months of intervention involving daily supplementation with multiple vitamins and minerals, an endoscopic survey was performed and squamous biopsies from 512 subjects were labeled with tritiated thymidine and autoradiographed. Analysis showed no treatment effect on the overall amount of squamous epithelial proliferation measured by the total labeling index. However, a measure of the vertical distribution of labeled cells showed lower values with supplementation: a 14% reduction in all subjects (P = 0.29), and a 29% reduction in nonsmokers (P = 0.03). These results suggest a potential modest benefit for short-term intervention with multiple vitamins and minerals on squamous epithelial cell proliferation of the esophagus in this high-risk population.
上皮细胞增殖异常被认为是胃肠道癌变的早期步骤。为了确定补充微量营养素是否可以减少食管鳞状上皮细胞的增殖，我们在中国林县开展了一项随机营养干预试验，评估了参与试验的受试者的细胞增殖情况。林县的食管癌发病率位居世界前列。经过30个月每日补充多种维生素和矿物质的干预后，进行了内镜检查，并对512名受试者的鳞状活检组织用氚标记胸腺嘧啶核苷进行标记，然后进行放射自显影。分析表明，通过总标记指数衡量的鳞状上皮细胞增殖总量没有治疗效果。然而，对标记细胞垂直分布的一项测量显示，补充后该值较低：所有受试者降低了14%（P = 0.29），非吸烟者降低了29%（P = 0.03）。这些结果表明，在这一高危人群中，短期补充多种维生素和矿物质对食管鳞状上皮细胞增殖可能有适度的潜在益处。
