Carrato C, Balague C, de Bolos C, Gonzalez E, Gambus G, Planas J, Perini J M, Andreu D, Real F X
Departament d'Immunologia, Universitat Autònoma de Barcelona, Spain.
Gastroenterology. 1994 Jul;107(1):160-72. doi: 10.1016/0016-5085(94)90073-6.
BACKGROUND/AIMS: The cloning of genes encoding human mucins is the basis for the study of their normal tissue distribution and the alterations associated with cancer. The aim of this study was to determine the normal and tumor tissue expression of MUC1, MUC2, MUC5B, and MUC5C.
The reactivity of apomucin-specific antibodies with fresh normal and tumor tissues was analyzed using immunohistochemical techniques.
Anti-MUC1 antibodies reacted with most glandular epithelia. Anti-MUC2 antibody was mainly reactive with intestinal goblet cells and cervical mucous cells. Anti-MUC5B was reactive with a wide range of epithelial tissues whereas anti-MUC5C was reactive with stomach, trachea, and endocervix. Double-labeling experiments showed coexpression of MUC1/MUC2 and MUC2/MUC5C in colonic tissue. Multiple apomucins were detected in colon cancers, but no relationship to histochemical mucus stains was observed.
It is concluded that (1) each apomucin shows a distinct tissue expression pattern; (2) multiple apomucins are present in a single tissue and at the single cell level; and (3) altered apomucin expression takes place in pathological colonic tissue.
背景/目的：克隆编码人黏蛋白的基因是研究其正常组织分布及与癌症相关改变的基础。本研究旨在确定MUC1、MUC2、MUC5B和MUC5C在正常组织和肿瘤组织中的表达情况。
采用免疫组织化学技术分析脱辅基黏蛋白特异性抗体与新鲜正常组织和肿瘤组织的反应性。
抗MUC1抗体与大多数腺上皮发生反应。抗MUC2抗体主要与肠杯状细胞和宫颈黏液细胞发生反应。抗MUC5B与多种上皮组织发生反应，而抗MUC5C与胃、气管和宫颈内膜发生反应。双重标记实验显示结肠组织中MUC1/MUC2和MUC2/MUC5C共表达。在结肠癌中检测到多种脱辅基黏蛋白，但未观察到与组织化学黏液染色的相关性。
得出以下结论：（1）每种脱辅基黏蛋白都表现出独特的组织表达模式；（2）单个组织和单个细胞水平上存在多种脱辅基黏蛋白；（3）病理性结肠组织中脱辅基黏蛋白表达发生改变。
