Ladinser B, Rossipal E, Pittschieler K
Department of Pediatrics, University of Graz, Austria.
Gut. 1994 Jun;35(6):776-8. doi: 10.1136/gut.35.6.776.
The ultra structural binding sites of endomysium antibodies have been studied on human umbilical cord tissue. The sensitivity and specificity of IgA endomysium antibodies were compared with recently described methods using basement membrane of smooth muscle of monkey oesophagus. Thirty adults affected by coeliac disease (10 in remission) and 75 healthy adult controls with normal intestinal mucosa (35 false antigliadin positive) were investigated. Sensitivity and correlation of endomysium antibodies with total villous atrophy in untreated coeliac disease patients were 100% on the human umbilical cord smooth muscles, and only 90% on the muscular layer of primate oesophagus. Indirect immunofluorescence was superior to peroxidase staining in detecting these IgA antibodies. The easy availability and enhanced testing sensitivity of the umbilical cord is an advance towards a better diagnostic tool for coeliac disease.
已在人脐带组织上研究了肌内膜抗体的超微结构结合位点。将IgA肌内膜抗体的敏感性和特异性与最近描述的使用猴食管平滑肌基底膜的方法进行了比较。对30名患有乳糜泻的成年人（10名处于缓解期）和75名肠道黏膜正常的健康成年对照者（35名抗麦胶蛋白假阳性）进行了研究。在未经治疗的乳糜泻患者中，肌内膜抗体与全绒毛萎缩的敏感性和相关性在人脐带平滑肌上为100%，而在灵长类动物食管肌层上仅为90%。在检测这些IgA抗体方面，间接免疫荧光优于过氧化物酶染色。脐带易于获取且检测敏感性增强，这是朝着更好的乳糜泻诊断工具迈出的一步。
