Gerna G, Steele A D, Hoshino Y, Sereno M, Garcia D, Sarasini A, Flores J
Virus Laboratory, University of Pavia, IRCCS Policlinico San Matteo, Italy.
J Gen Virol. 1994 Jul;75 ( Pt 7):1781-4. doi: 10.1099/0022-1317-75-7-1781.
The sequences of the gene encoding VP7 (the major outer capsid protein) from one bovine and three human rotavirus strains were determined because of their unusual VP7 specificities. Two of the human strains (PA 169 and PA 151) had VP7 serotype 6 specificity whereas the two other strains, recovered from a child (HAL 1166) and a calf (678) belonged to VP7 serotype 8. The serotype 8 strains exhibited a high degree of sequence conservation when compared with each other and with other serotype 8 strains previously sequenced. The serotype 6 human strains shared a greater degree of sequence similarity with previously reported serotype 6 bovine strains than with other rotavirus serotypes; however the degree of sequence similarity among PA 169, PA 151 and the bovine strains was lower than had been previously reported for strains belonging to the same serotype. The demonstration of rotavirus serotypes that are shared between human and animal species supports the concept that interspecies transmission occurs and may play a role in rotavirus evolution.
由于其不寻常的VP7特异性，对一株牛轮状病毒和三株人轮状病毒编码VP7（主要外衣壳蛋白）的基因序列进行了测定。两株人源毒株（PA 169和PA 151）具有VP7血清型6特异性，而另外两株分别从一名儿童（HAL 1166）和一头小牛（678）中分离出的毒株属于VP7血清型8。血清型8毒株彼此之间以及与之前测序的其他血清型8毒株相比，表现出高度的序列保守性。血清型6人源毒株与之前报道的血清型6牛源毒株的序列相似性程度高于与其他轮状病毒血清型；然而，PA 169、PA 151与牛源毒株之间的序列相似性程度低于之前报道的属于同一血清型的毒株。人和动物物种间共有的轮状病毒血清型的证明支持了种间传播发生且可能在轮状病毒进化中起作用的概念。
