Chang K O, Parwani A V, Saif L J
Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, Ohio State University, Wooster, USA.
Arch Virol. 1995;140(7):1279-83. doi: 10.1007/BF01322753.
The prevalent G serotypes of group A bovine rotavirus (BRV) reported are G6, G10, and less commonly, G8. Neonatal Calf Diarrhea Virus (NCDV), Lincoln and Cody strains were first isolated from diarrheic calves in Nebraska. The NCDV Lincoln strain is the currently used U.S. vaccine strain and has a G6 serotype. In this study, the complete nucleotide sequence of the VP7 gene of NCDV Cody (I-801 strain) was determined using the primer extension method. The VP7 gene nucleotide sequence homologies between Cody I-801 and established G8 rotaviruses, A5 (Thailand BRV), 678 (UK BRV), B37 (human RV) and 69M (human RV) were 84.7%, 86.4%, 84.7% and 85.9%, respectively. The deduced VP7 amino acid sequence of Cody I-801 was similar to that of A5, 678, B37 and 69M (93.6%, 95.7%, 92.6% and 95.1%, respectively). The VP7 gene nucleic acid sequence homologies between NCDV Cody (I-801) and NCDV Lincoln or B223 (G10) was 76.2% and the deduced VP7 amino acid sequence homologies between Cody I-801 and NCDV Lincoln or B223 were 82.5% and 81.3%, respectively. Thus, our sequence data suggests that the VP7 gene of Cody I-801 strain of BRV is genetically most similar to G8 rotaviruses and unrelated to the NCDV Lincoln G6 rotavirus strain.
报道的A组牛轮状病毒（BRV）流行G血清型为G6、G10，较少见的是G8。新生犊牛腹泻病毒（NCDV）、林肯株和科迪株最初是从内布拉斯加州腹泻犊牛中分离出来的。NCDV林肯株是目前美国使用的疫苗株，具有G6血清型。在本研究中，采用引物延伸法测定了NCDV科迪株（I-801株）VP7基因的完整核苷酸序列。科迪I-801株与已确定的G8轮状病毒A5（泰国BRV）、678（英国BRV）、B37（人RV）和69M（人RV）的VP7基因核苷酸序列同源性分别为84.7%、86.4%、84.7%和85.9%。科迪I-801株推导的VP7氨基酸序列与A5、678、B37和69M株相似（分别为93.6%、95.7%、92.6%和95.1%）。NCDV科迪株（I-801）与NCDV林肯株或B223株（G10）的VP7基因核酸序列同源性为76.2%，科迪I-801株与NCDV林肯株或B223株推导的VP7氨基酸序列同源性分别为82.5%和81.3%。因此，我们的序列数据表明，BRV科迪I-801株的VP7基因在遗传上与G8轮状病毒最相似，与NCDV林肯G6轮状病毒株无关。
