Tate M L, Goosen G J, Patene H, Pearse A J, McEwan K M, Fennessy P F
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
J Hered. 1997 Sep-Oct;88(5):361-5. doi: 10.1093/oxfordjournals.jhered.a023119.
Interspecies hybrids provide unique opportunities for fundamental genetic analyses and for genetic improvement of farmed deer. We have bred F1 hybrids by artificial insemination of red deer hinds (Cervus elaphus) with semen from Père David's deer (Elaphurus davidianus). The male and female F1 Père David deer x red deer hybrids were fertile and in matings with red deer have produced over 300 viable backcross hybrids. DNA was collected from the backcross progeny as an international reference panel for gene linkage mapping and to investigate associations between segregating species-specific genetic markers and phenotypic traits. We have measured a range of phenotypic traits in the backcross hybrids and red deer. Several traits appear suitable for genetic analysis using mapped genetic markers, including gestation length, growth rate, live weight, head morphometrics, and tail length. Typically these traits show a large difference between Père David's deer and red deer and a high variance in the backcross so that many individuals have a phenotype outside the range observed in red deer.
种间杂交为基础遗传学分析和养殖鹿的遗传改良提供了独特机遇。我们通过用麋鹿（Elaphurus davidianus）的精液对马鹿（Cervus elaphus）母鹿进行人工授精培育出了F1代杂种。F1代雄性和雌性麋鹿×马鹿杂种具有生育能力，与马鹿交配产生了300多个可存活的回交杂种。从回交后代中收集DNA，作为基因连锁图谱绘制的国际参考面板，并研究分离的物种特异性遗传标记与表型性状之间的关联。我们测量了回交杂种和马鹿的一系列表型性状。包括妊娠期长度、生长速率、活体重、头部形态测量和尾长在内的几个性状似乎适合使用定位的遗传标记进行遗传分析。通常，这些性状在麋鹿和马鹿之间表现出很大差异，在回交中具有高变异性，因此许多个体的表型超出了马鹿所观察到的范围。
