Scolnik M, Konichezky M, Tykochinsky G, Servadio C, Abramovici A
Laboratory of Developmental Pathology, Beilinson Medical Center, Petach Tikva, Israel.
Prostate. 1994 Jul;25(1):1-9. doi: 10.1002/pros.2990250102.
The vasoactive effect of a fragrance compound, citral, on rat ventral prostate is presented. A combined method of Indian-ink perfusion and postfixation transparency was used. One single dose of citral applied on the back skin of the rat induced an immediate triple response-like effect on the prostatic vascular bed. A slight vasodilatation appeared already after 2 1/2 min, followed by a marked vasoconstriction process after 5-min postcitral administration. Toward the period of 10 min, a second vasodilatation process was noted which persisted for the following 24 hr of observation. This study has demonstrated that changes in the microvascularization of the rat prostate can be estimated by counting the number of carbon-stained blood vessels. The results obtained by this technique are closely related with the findings in the vascular network profile of the macroangiographic observations. The mechanism of action of citral on the microvascularization of the rat ventral prostate is yet unknown. Based on the present findings, we suggest that the neoplastic capacity of citral upon the prostatic epithelia is activated via a nonspecific inflammatory reaction modulated either by local release of neurotransmitters or throughout a direct effect of citral on the endothelial cells.
本文介绍了一种香料化合物柠檬醛对大鼠腹侧前列腺的血管活性作用。采用了印度墨水灌注和固定后透明化的联合方法。在大鼠背部皮肤涂抹单次剂量的柠檬醛后，前列腺血管床立即出现类似三联反应的效应。在给予柠檬醛后2.5分钟，即出现轻微的血管舒张，随后在5分钟时出现明显的血管收缩过程。到10分钟时，观察到第二次血管舒张过程，并在接下来的24小时观察期内持续存在。本研究表明，通过计数碳染色血管的数量可以评估大鼠前列腺微血管化的变化。通过该技术获得的结果与宏观血管造影观察中血管网络轮廓的发现密切相关。柠檬醛对大鼠腹侧前列腺微血管化的作用机制尚不清楚。基于目前的研究结果，我们认为柠檬醛对前列腺上皮细胞的致癌能力是通过神经递质的局部释放或柠檬醛对内皮细胞的直接作用所调节的非特异性炎症反应而被激活的。
