Geldof A A, Engel C, Rao B R
Department of Endocrinology, Academisch Ziekenhuis, Vrije Universiteit, Amsterdam, The Netherlands.
Urol Res. 1992;20(2):139-44. doi: 10.1007/BF00296526.
A rat model for benign prostatic hyperplasia in man (BPH) was investigated. Citral treatment of male Copenhagen rats for 4 months via the transdermal route resulted in a marked hyperplasia of glandular epithelium and interglandular stroma in the ventral prostate. Despite the cellular hyperplasia there was not a significant increase in prostate weight. Investigations of the mechanism of action of citral showed that application of citral directly to the vagina in female, ovariectomized rats resulted in an increased proliferation of vaginal epithelium and a significant increase in the BrdUrd incorporation in vaginal epithelial cells, in short a similar effect to that of estrogen application. In an in vitro assay citral proved to inhibit estrogen binding to estrogen receptors, while no such inhibition was observed with testosterone for androgen receptors. These observations together with the estrogen implication in the BPH and the reported incidence of gynecomastia following exposure to geraniol, a precursor of citral, strongly suggest that the prostatic hyperplasia-inducing capacity of citral may be due to its estrogenic action.
对一种人类良性前列腺增生（BPH）大鼠模型进行了研究。通过经皮途径对雄性哥本哈根大鼠进行4个月的柠檬醛治疗，导致腹侧前列腺的腺上皮和腺间基质显著增生。尽管细胞增生，但前列腺重量没有显著增加。对柠檬醛作用机制的研究表明，将柠檬醛直接应用于雌性去卵巢大鼠的阴道，会导致阴道上皮细胞增殖增加，且阴道上皮细胞中溴脱氧尿苷（BrdUrd）掺入显著增加，简而言之，这与应用雌激素的效果相似。在体外试验中，柠檬醛被证明可抑制雌激素与雌激素受体的结合，而对于雄激素受体，睾酮未观察到这种抑制作用。这些观察结果，连同雌激素在BPH中的作用以及接触柠檬醛前体香叶醇后报道的男性乳腺增生发病率，强烈表明柠檬醛诱导前列腺增生的能力可能归因于其雌激素作用。
