Coursaget P, Depril N, Buisson Y, Molinié C, Roué R
Institut de Virologie de Tours, Faculté de Pharmacie, Tours, France.
Res Virol. 1994 Jan-Feb;145(1):51-7. doi: 10.1016/s0923-2516(07)80007-0.
Four synthetic peptides were used to develop an ELISA for detecting hepatitis type E infections. Antibodies to HEV were detected in 54 of 64 patients present during two outbreaks previously recognized as being due to hepatitis E virus. Those patients included French soldiers stationed in Chad at the time, and Algerian civilians residing in Algeria and considered as positive controls. Anti-HEV were also detected in 3 out of 74 cases of sporadic non-A, non-B, non-C hepatitis in France, and in 2 out of 150 anti-HAV-IgM-positive sporadic cases, also in France, but in none of 278 healthy adults from the general French population. Among a total of 34 anti-HEV-positive cases in French subjects, 32 were shown to be associated with travel outside of western Europe; in two cases, however, HEV transmission appeared to have taken place in Europe. Using this ELISA, a rapid drop in anti-HEV antibodies to undetectable levels following the acute phase of the disease was observed in a high proportion of the infected subjects. Thus, the present test would appear to be more suitable for diagnostic purposes than for epidemiological investigations.
使用四种合成肽开发了一种用于检测戊型肝炎感染的酶联免疫吸附测定法（ELISA）。在先前认定为由戊型肝炎病毒引起的两次疫情期间出现的64名患者中，有54名检测到了抗戊型肝炎病毒抗体。这些患者包括当时驻扎在乍得的法国士兵以及居住在阿尔及利亚的阿尔及利亚平民，他们被视为阳性对照。在法国的74例散发性非甲、非乙、非丙型肝炎病例中，有3例检测到了抗戊型肝炎病毒抗体；在法国的150例抗甲型肝炎病毒IgM阳性散发病例中，也有2例检测到了抗戊型肝炎病毒抗体，但在278名法国普通人群的健康成年人中均未检测到。在法国受试者中总共34例抗戊型肝炎病毒抗体阳性病例中，有32例显示与西欧以外的旅行有关；然而，在两例病例中，戊型肝炎病毒传播似乎发生在欧洲。使用这种ELISA法，在很大一部分感染受试者中观察到，疾病急性期后抗戊型肝炎病毒抗体迅速下降至无法检测的水平。因此，目前的检测方法似乎更适合用于诊断目的，而非流行病学调查。
