Wronski T J, Yen C F
Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville.
Bone. 1994 Jan-Feb;15(1):51-8. doi: 10.1016/8756-3282(94)90891-5.
The purpose of this study was to determine whether PTH has anabolic effects on cortical bone in ovariectomized (OVX) rats similar to those previously observed in cancellous bone. Groups of OVX rats were untreated for the first 4 weeks postovariectomy followed by treatment for 5-, 10-, or 15-week periods with vehicle, estrogen, the bisphosphonate rise-dronate (NE-58095), PTH, PTH+estrogen or PTH+NE-58095. A group of sham-operated control rats was treated with vehicle alone. Cross sections of the tibial diaphysis immediately proximal to the tibiofibular junction were subjected to quantitative bone histomorphometry. In comparison to vehicle-treated control rats, the following structural changes were detected in cortical bone of OVX rats after 15 weeks of vehicle treatment: increased cortical bone tissue area, increased cortical bone area, and a trend for increased bone marrow area. Indices of periosteal bone formation were increased in vehicle-treated OVX rats at 5 weeks, but declined to control levels at the later time points. In addition, vehicle-treated OVX rats exhibited a nonsignificant trend for increased endocortical bone formation relative to vehicle-treated control rats throughout most of the study. Estrogen, but not NE-58095, prevented most of the changes in cortical bone structure associated with ovariectomy. In comparison to vehicle treatment of OVX rats, estrogen decreased both periosteal and endocortical bone formation whereas NE-58095 decreased endocortical, but not periosteal, bone formation. Treatment of OVX rats with PTH alone increased cortical bone area and width as well as decreased bone marrow area compared to vehicle-treated OVX rats.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是确定甲状旁腺激素（PTH）对去卵巢（OVX）大鼠皮质骨是否具有合成代谢作用，类似于先前在松质骨中观察到的作用。将OVX大鼠分组，在卵巢切除术后的前4周不进行治疗，随后分别用赋形剂、雌激素、双膦酸盐利塞膦酸钠（NE-58095）、PTH、PTH + 雌激素或PTH + NE-58095进行5周、10周或15周的治疗。一组假手术对照大鼠仅用赋形剂治疗。对胫腓关节近端紧邻的胫骨骨干横断面进行定量骨组织形态计量学分析。与赋形剂治疗的对照大鼠相比，在赋形剂治疗15周后，OVX大鼠的皮质骨出现以下结构变化：皮质骨组织面积增加、皮质骨面积增加以及骨髓面积有增加趋势。在5周时，赋形剂治疗的OVX大鼠骨膜骨形成指标增加，但在随后的时间点降至对照水平。此外，在整个研究的大部分时间里，赋形剂治疗的OVX大鼠相对于赋形剂治疗的对照大鼠，其骨内膜骨形成有不显著的增加趋势。雌激素而非NE-58095可预防与卵巢切除相关的皮质骨结构的大部分变化。与OVX大鼠的赋形剂治疗相比，雌激素可降低骨膜和骨内膜骨形成，而NE-58095可降低骨内膜而非骨膜骨形成。与赋形剂治疗的OVX大鼠相比，单独用PTH治疗OVX大鼠可增加皮质骨面积和宽度，并减少骨髓面积。（摘要截短至250字）
