Cohen M B, Koener J F, Feyereisen R
Department of Entomology, University of Arizona, Tucson 85721.
Gene. 1994 Sep 2;146(2):267-72. doi: 10.1016/0378-1119(94)90304-2.
We determined the sequence of a cytochrome P450-encoding gene, CYP6A1, in an insecticide-resistant strain (Rutgers) and in insecticide-susceptible strains (aabys and sbo) of the house fly Musca domestica. The deduced amino acid (aa) sequence of CYP6A1 is 98% identical between Rutgers and aabys, and it is identical between Rutgers and sbo. Differences in aa sequence occur in regions that are not thought to participate in the active site. CYP6A1 has a single intron, 60 bp in length. It does not occur in a position homologous to intron sites in genes of the related CYP3 family or other vertebrate P450 families. We mapped the transcription start point (tsp), TATA box and barbie box [Shaw and Fulco, J. Biol. Chem. 269 (1993) 2997-3004] of this phenobarbital-inducible gene. Although overexpression of CYP6A1 in strain Rutgers is controlled by a locus on chromosome II [Cariño et al., Insect Biochem. Mol. Biol. 24 (1994) 411-418], we have localized CYP6A1 to chromosome V, thus showing that a trans-acting factor controls the overexpression of the gene in the Rutgers strain.
我们测定了家蝇抗药性品系（罗格斯品系）和敏感品系（阿比品系和斯波品系）中编码细胞色素P450的基因CYP6A1的序列。罗格斯品系和阿比品系之间CYP6A1推导的氨基酸序列有98%相同，罗格斯品系和斯波品系之间则完全相同。氨基酸序列的差异出现在被认为不参与活性位点的区域。CYP6A1有一个60bp长的单一内含子。它所处位置与相关CYP3家族基因或其他脊椎动物P450家族基因的内含子位点不同源。我们确定了这个苯巴比妥诱导基因的转录起始点（tsp）、TATA盒和芭比盒[Shaw和Fulco，《生物化学杂志》269(1993)2997 - 3004]。虽然罗格斯品系中CYP6A1的过表达受二号染色体上一个位点的控制[卡里尼奥等人，《昆虫生物化学与分子生物学》24(1994)411 - 418]，但我们已将CYP6A1定位到五号染色体，从而表明一个反式作用因子控制着该基因在罗格斯品系中的过表达。
