粗糙脉孢菌中磷酸盐代谢的调控：可阻遏碱性磷酸酶结构基因的鉴定。
Regulation of phosphate metabolism in Neurospora crassa: identification of the structural gene for repressible alkaline phosphatase.
作者信息
Lehman J F, Metzenberg R L
出版信息
Genetics. 1976 Oct;84(2):175-82. doi: 10.1093/genetics/84.2.175.
Abstract
Five additional mutants of Neurospora crassa have been isolated that lack the repressible alkaline phosphatase. The mutations in these strains map at a previously assigned locus on Linkage Group V designated pho-2 (GLEASON and METZENBERG 1974). The five new mutants, as well as three previously isolated by GLEASON and METZENBERG (1974), were examined for the presence of cross-reacting material to antibody prepared against purified wild-type enzyme. Two of the mutants produced high levels of cross-reacting material, thus providing evidence that the pho-2 locus includes the structural gene for the repressible alkaline phosphatase. Two revertants were obtained from one of the mutants that contained cross-reacting material. Neither revertant produced an enzyme that could be distinguished physicochemically from that of wild type. A method for measuring very low levels of repressible alkaline phosphatase in crude extracts is also described.
摘要
已分离出另外五个粗糙脉孢菌突变体，它们缺乏可阻遏的碱性磷酸酶。这些菌株中的突变位于V连锁群上一个先前指定的基因座，命名为pho-2（格里森和梅岑伯格，1974年）。检测了这五个新突变体以及格里森和梅岑伯格（1974年）先前分离出的三个突变体，看它们是否存在与针对纯化野生型酶制备的抗体发生交叉反应的物质。其中两个突变体产生了高水平的交叉反应物质，从而证明pho-2基因座包含可阻遏碱性磷酸酶的结构基因。从其中一个含有交叉反应物质的突变体中获得了两个回复体。这两个回复体产生的酶在物理化学性质上都无法与野生型酶区分开来。还描述了一种测量粗提物中极低水平可阻遏碱性磷酸酶的方法。
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Regulation of phosphate metabolism in Neurospora crassa: isolation of mutants deficient in ther repressible alkaline phosphatase.粗糙脉孢菌中磷酸盐代谢的调控：可阻遏碱性磷酸酶缺陷型突变体的分离
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