拥挤诱导的细胞骨架元件组织：II. 帽蛋白对自发形成的丝束的溶解作用
Crowding-induced organization of cytoskeletal elements: II. Dissolution of spontaneously formed filament bundles by capping proteins.
作者信息
Madden T L, Herzfeld J
机构信息
Department of Chemistry, Brandeis University, Waltham, Massachusetts 02254-9110.
出版信息
J Cell Biol. 1994 Jul;126(1):169-74. doi: 10.1083/jcb.126.1.169.
Abstract
Through calculations of molecular packing constraints in crowded solutions, we have previously shown that dispersions of filament forming proteins and soluble proteins can be unstable at physiological concentrations, such that tight bundles of filaments are formed spontaneously, in the absence of any accessory binding proteins. Here we consider the modulation of this phenomenon by capping proteins. The theory predicts that, by shortening the average filament length, capping alleviates the packing problem. As a result, the dispersed isotropic solution is stable over an expanded range of compositions.
摘要
通过计算拥挤溶液中的分子堆积限制，我们先前已经表明，丝状形成蛋白和可溶性蛋白的分散体在生理浓度下可能是不稳定的，以至于在没有任何辅助结合蛋白的情况下，会自发形成紧密的丝状束。在这里，我们考虑封端蛋白对这种现象的调节作用。该理论预测，通过缩短平均丝长度，封端可以缓解堆积问题。因此，分散的各向同性溶液在更广泛的组成范围内是稳定的。
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