奥美拉唑对幽门螺杆菌的作用及其与菌株毒性的关系。
Activity of omeprazole on Helicobacter pylori and relation to toxicity of strains.
作者信息
Figura N, Armellini D, Bugnoli M, Bayeli P F, Gennari C, Crabtree J E
机构信息
Istituto di Patologia Speciale Medica, University of Siena, Policlinico Le Scotte, Italy.
出版信息
J Clin Pathol. 1994 May;47(5):440-2. doi: 10.1136/jcp.47.5.440.
AIMS
To see whether the activity of omeprazole on Helicobacter pylori is associated with toxicity of strains; to determine whether omeprazole inhibited vacuolisation of cells in culture induced by H pylori cytotoxin and by ureas, and if omeprazole prevented H pylori motility.
METHODS
Minimal inhibitory concentrations (MICs) of omeprazole were determined for seven cytotoxic and five non-cytotoxic H pylori strains. Omeprazole at different concentrations was incubated with cytotoxic and non-cytotoxic extracts of H pylori, or with purified H pylori urease, and added to cells in culture. Inhibition of motility by omeprazole was tested in semi-solid medium.
RESULTS
MIC90 of omeprazole was 40 micrograms/ml. MICs for cytotoxic and noncytotoxic organisms were similar. Omeprazole did not prevent vacuolisation induced by the cytotoxic extract, but at high concentrations it inhibited the formation of vacuoles induced by urease. Motility was not inhibited by the drug.
CONCLUSIONS
H pylori cytotoxin is not the target of the antimicrobial activity of omeprazole. Should the drug reach clinically effective concentrations in vivo, it could potentially prevent the mucosal damage caused by the vacuolising activity of urease.
目的
观察奥美拉唑对幽门螺杆菌的活性是否与菌株毒性相关；确定奥美拉唑是否抑制幽门螺杆菌细胞毒素和尿素诱导的培养细胞空泡化，以及奥美拉唑是否阻止幽门螺杆菌运动。
方法
测定奥美拉唑对7株细胞毒性和5株非细胞毒性幽门螺杆菌菌株的最低抑菌浓度（MIC）。将不同浓度的奥美拉唑与幽门螺杆菌的细胞毒性和非细胞毒性提取物或纯化的幽门螺杆菌脲酶一起孵育，并添加到培养的细胞中。在半固体培养基中测试奥美拉唑对运动的抑制作用。
结果
奥美拉唑的MIC90为40微克/毫升。细胞毒性和非细胞毒性菌株的MIC相似。奥美拉唑不能阻止细胞毒性提取物诱导的空泡化，但在高浓度时它抑制脲酶诱导的空泡形成。该药物不抑制运动。
结论
幽门螺杆菌细胞毒素不是奥美拉唑抗菌活性的靶点。如果该药物在体内达到临床有效浓度，它可能会预防脲酶空泡化活性引起的粘膜损伤。
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