Hardoff D, Levanon D, Gitay H, Nir I
J Pediatr Gastroenterol Nutr. 1986 Jul-Aug;5(4):560-4. doi: 10.1097/00005176-198607000-00009.
Biopsies from jejunum of nonceliac patients and patients with active celiac disease were studied and compared by means of transmission (TEM) and scanning (SEM) electron microscopy. Fracturing of dried specimens enabled the visualization of the epithelial microvilli with SEM. Alteration in microvillous appearance in celiac patients, as determined by TEM, could also be clearly detected in SEM images. The SEM preparation is easier to accomplish and less time-consuming compared to the TEM. This may be an advantage for clinical assessment of jejunal biopsies in syndromes in which early detection of alterations in microvilli in the epithelial mucosa is of diagnostic value.
对非乳糜泻患者和活动性乳糜泻患者空肠的活检样本进行了研究，并通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）进行比较。干燥标本的断裂处理使扫描电子显微镜能够观察到上皮微绒毛。通过透射电子显微镜确定的乳糜泻患者微绒毛外观的改变，在扫描电子显微镜图像中也能清晰检测到。与透射电子显微镜相比，扫描电子显微镜的样本制备更容易完成且耗时更少。对于上皮黏膜微绒毛改变早期检测具有诊断价值的综合征，这可能是对空肠活检进行临床评估的一个优势。
