Monckton D G, Jeffreys A J
Department of Genetics, University of Leicester, UK.
Nucleic Acids Res. 1994 Jun 11;22(11):2155-7. doi: 10.1093/nar/22.11.2155.
Minisatellite isoallelism, i.e. the occurrence of minisatellite alleles with different internal sequence composition but indistinguishable length, is a common limitation of minisatellite allele length analysis. Internal sequence variation can be used to distinguish such isoalleles, provided that detailed sequence knowledge of its basis is available. We now show that minisatellite isoalleles can also be simply resolved by single-stranded conformational polymorphisms (SSCP) arising during agarose gel electrophoresis. SSCP on agarose gels can be used to distinguish minisatellite isoalleles either after PCR amplification, or by standard Southern blot analysis of genomic DNA.
小卫星等位基因异质性，即出现内部序列组成不同但长度无法区分的小卫星等位基因，是小卫星等位基因长度分析的常见限制。如果能够获得其基础的详细序列信息，内部序列变异可用于区分此类异等位基因。我们现在表明，小卫星异等位基因也可以通过琼脂糖凝胶电泳过程中产生的单链构象多态性（SSCP）简单地进行分辨。琼脂糖凝胶上的SSCP可用于在PCR扩增后或通过对基因组DNA进行标准Southern印迹分析来区分小卫星异等位基因。
