唾液酸在红细胞存活中的作用。

Role of sialic acid in erythrocyte survival.

作者信息

Durocher J R, Payne R C, Conrad M E

机构信息

Department of Hematology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20012.

出版信息

Blood. 1975 Jan;45(1):11-20.

PMID:803103

Abstract

The role of membrane sialic acid in erythrocyte survival is unclear, although there is evidence for a reduction in sialic acid and surface charge in older erythrocytes. We reduced the surface charge of human, rat, and rabbit erythrocytes by removing sialic acid with neuraminidase. Reduction in sialic acid correlated with decreases in electrophoretic mobility and loss of PAS staining of membrane glycoproteins on polyacrylamide gels. No changes in ATP levels or deformability were found. 51Cr erythrocyte survivals in rats and rabbits showed rapid clearance of desialylated erythrocytes with sequestration by the liver. These results suggest that reduction in erythrocyte sialic acid is a mechanism of erythrocyte destruction and may be important in erythrocyte senescence.

摘要

膜唾液酸在红细胞存活中的作用尚不清楚，尽管有证据表明衰老红细胞中的唾液酸和表面电荷会减少。我们用神经氨酸酶去除唾液酸，从而降低了人、大鼠和兔红细胞的表面电荷。唾液酸的减少与电泳迁移率的降低以及聚丙烯酰胺凝胶上膜糖蛋白PAS染色的丧失相关。未发现ATP水平或可变形性有变化。大鼠和兔的51Cr红细胞存活实验表明，去唾液酸红细胞会被肝脏滞留并迅速清除。这些结果表明，红细胞唾液酸的减少是红细胞破坏的一种机制，可能在红细胞衰老中起重要作用。