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弯曲乳杆菌FS47产生的一种热稳定细菌素——弯曲菌素FS47的纯化及部分氨基酸序列分析

Purification and partial amino acid sequence of curvaticin FS47, a heat-stable bacteriocin produced by Lactobacillus curvatus FS47.

作者信息

Garver K I, Muriana P M

机构信息

Department of Food Science, Purdue University, West Lafayette, Indiana 47907.

出版信息

Appl Environ Microbiol. 1994 Jun;60(6):2191-5. doi: 10.1128/aem.60.6.2191-2195.1994.

DOI:10.1128/aem.60.6.2191-2195.1994
PMID:8031103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC201625/
Abstract

Curvaticin FS47, a bacteriocin produced by Lactobacillus curvatus FS47, is inhibitory to Listeria monocytogenes, as well as Lactobacillus, Pediococcus, Enterococcus, and Bacillus spp. The bacteriocin was purified by 40% ammonium sulfate precipitation, solid-phase extraction, and reversed-phase high-pressure liquid chromatography. Purified curvaticin FS47 was determined to be 4.07 kDa by mass spectrometry and was partially sequenced. Thirty-one N-terminal amino acids were identified; the curvaticin FS47 protein sequence did not show homology to the pediocin-like group of bacteriocins.

摘要

弯曲乳杆菌FS47产生的细菌素Curvaticin FS47对单核细胞增生李斯特菌以及乳杆菌属、片球菌属、肠球菌属和芽孢杆菌属具有抑制作用。该细菌素通过40%硫酸铵沉淀、固相萃取和反相高压液相色谱法进行纯化。通过质谱法测定纯化后的Curvaticin FS47分子量为4.07 kDa,并进行了部分测序。鉴定出了31个N端氨基酸;Curvaticin FS47蛋白序列与类片球菌素细菌素组没有同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2a/201625/a7880ec1a7c9/aem00023-0499-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2a/201625/77a8509c4a75/aem00023-0499-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2a/201625/a7880ec1a7c9/aem00023-0499-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2a/201625/77a8509c4a75/aem00023-0499-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db2a/201625/a7880ec1a7c9/aem00023-0499-b.jpg

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