Prato Murphy M L, Meyer R F, Mebus C, Schudel A A, Rodriguez M
Institute of Virology, CICV-INTA, Moron, Argentina.
Arch Virol. 1994;136(3-4):299-307. doi: 10.1007/BF01321059.
This study was undertaken in order to explore possible sites of foot-and-mouth disease virus (FMDV) persistence during the carrier state. Tissue samples taken from experimentally infected animals at different times post-infection (p.i.) were examined by conventional viral isolation and the polymerase chain reaction (PCR) technique. The analysis of samples from several organs taken from 17 bovines between 3 and 270 days p.i. allowed the following conclusions: 1) Virus present in oesophageal-pharyngeal fluids (OPF) during the carrier state originates in the pharynx as shown by the detection of antisense FMDV RNA by PCR, 2) PCR is more sensitive than standard virus isolation techniques and may be used for the rapid detection of FMDV in specimens obtained during the acute stage of FMD and for identification of persistently infected cattle.
本研究旨在探索口蹄疫病毒（FMDV）在携带状态下可能的持续存在部位。通过传统病毒分离和聚合酶链反应（PCR）技术，对感染后不同时间（p.i.）从实验感染动物采集的组织样本进行了检测。对17头奶牛在感染后3至270天从多个器官采集的样本进行分析后得出以下结论：1）携带状态下存在于食管-咽液（OPF）中的病毒起源于咽部，这可通过PCR检测到反义FMDV RNA得以证明；2）PCR比标准病毒分离技术更敏感，可用于口蹄疫急性期采集的标本中FMDV的快速检测以及持续感染牛的鉴定。
