光滑念珠菌的异源基因表达与蛋白质分泌

Heterologous gene expression and protein secretion from Candida glabrata.

作者信息

Macreadie I G, Castelli L A, Mehra R K, Thorvaldsen J L, Winge D R

机构信息

Biomolecular Research Institute, Parkville, Victoria, Australia.

出版信息

Biotechnol Appl Biochem. 1994 Jun;19(3):265-9.

PMID:8031503

Abstract

We have examined heterologous protein secretion from Candida glabrata with the aid of a stable C. glabrata vector and a secretion reporter cassette comprising the Saccharomyces cerevisiae PGK-gene promoter and a Kluveromyces Iactis secretion signal to drive secretion of Escherichia coli beta-lactamase. Abundant secretion of beta-lactamase from C. glabrata indicates that the S. cerevisiae PGK promoter functions in C. glabrata. Furthermore, we show that C. glabrata processes the secreted beta-lactamase in a manner similar to, but not identical with, S. cerevisiae and K. lactis. C. glabrata may be a suitable new host for the expression of foreign genes.

摘要

我们借助一种稳定的光滑念珠菌载体和一个分泌报告盒，对光滑念珠菌的异源蛋白分泌进行了研究。该分泌报告盒包含酿酒酵母PGK基因启动子和乳酸克鲁维酵母分泌信号，用于驱动大肠杆菌β-内酰胺酶的分泌。光滑念珠菌大量分泌β-内酰胺酶，这表明酿酒酵母PGK启动子在光滑念珠菌中发挥作用。此外，我们发现光滑念珠菌处理分泌的β-内酰胺酶的方式与酿酒酵母和乳酸克鲁维酵母相似，但并不完全相同。光滑念珠菌可能是表达外源基因的合适新宿主。