Balsara B R, Borges A M, Pradhan S A, Rajpal R M, Bhisey A N
Cancer Research Institute, Tata Memorial Hospital, Tata Memorial Centre, Parel, Bombay, India.
Eur J Cancer B Oral Oncol. 1994;30B(2):98-101. doi: 10.1016/0964-1955(94)90060-4.
DNA content was measured in 68 squamous cell carcinomas (SCC) of the oral cavity by flow cytometry. Samples fixed in 95% alcohol and disaggregated with 0.5% pepsin were stained with 4,6-diamidino-phenylindole (DAPI) for flow cytometry. The tumours were classified according to the TNM classification--1987, and graded histopathologically. A positive correlation between tumour size and ploidy status was observed. Poorly differentiated tumours were mainly non-diploid (P < 0.01, chi 2). A majority of the node positive (N+) tumours were non-diploid (P < 0.05). It was possible to distinguish diploid, N+, T4 tumours from diploid, N-, T4 tumours by their higher S-phase fraction (SpF). SpF was also a useful parameter to differentiate diploid, N+ tumours from diploid, N- tumours among the moderately differentiated (MD) SCC. These results suggest that ploidy and SpF can be useful correlates of tumour behaviour.
通过流式细胞术对68例口腔鳞状细胞癌（SCC）的DNA含量进行了测定。将固定于95%酒精中并用0.5%胃蛋白酶解离的样本用4,6-二脒基-2-苯基吲哚（DAPI）染色，用于流式细胞术检测。肿瘤根据TNM分类-1987进行分类，并进行组织病理学分级。观察到肿瘤大小与倍体状态之间存在正相关。低分化肿瘤主要为非二倍体（P<0.01，卡方检验）。大多数淋巴结阳性（N+）肿瘤为非二倍体（P<0.05）。通过较高的S期分数（SpF），可以将二倍体、N+、T4肿瘤与二倍体、N-、T4肿瘤区分开来。在中度分化（MD）的SCC中，SpF也是区分二倍体、N+肿瘤与二倍体、N-肿瘤的有用参数。这些结果表明，倍体和SpF可能是肿瘤行为的有用相关指标。
