Bridge P M, Ball D J, Mackinnon S E, Nakao Y, Brandt K, Hunter D A, Hertl C
Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.
Exp Neurol. 1994 Jun;127(2):284-90. doi: 10.1006/exnr.1994.1104.
Nerve crush is a commonly used experimental model in the rat; however, a standard method of inducing this injury has not been defined. This study examined six crush techniques that are frequently used and characterized the subsequent nerve injury. Five types of nerve crush using a No. 5 jeweler's forceps and a sixth using a 30-s single crush with a serrated hemostat were studied in the posterior tibial nerve of the Lewis rat. Regeneration was evaluated using serial walking track assessments at 1, 2, 4, 6, and 8 weeks postoperatively. Nerve conduction studies and histological examination were performed at 2 days, 2 weeks, and 8 weeks. Blood-nerve barrier breakdown was observed at 2 days and recovered by 2 weeks. By 4 weeks normal walking track patterns were obtained in all groups. A pattern of Wallerian degeneration and axonal regeneration was noted at 2 weeks, with histological recovery in all groups by 8 weeks. Nerve crush, induced by any of the six methods tested, was similar and provides a reliable model of axonotmesis.
神经挤压是大鼠中常用的实验模型；然而，尚未定义诱导这种损伤的标准方法。本研究检查了六种常用的挤压技术，并对随后的神经损伤进行了特征描述。在Lewis大鼠的胫后神经中研究了五种使用5号珠宝钳的神经挤压类型，以及第六种使用带锯齿止血钳进行30秒单次挤压的类型。术后1、2、4、6和8周使用连续步行轨迹评估来评估再生情况。在术后2天、2周和8周进行神经传导研究和组织学检查。在2天时观察到血-神经屏障破坏，并在2周时恢复。到4周时，所有组均获得正常的步行轨迹模式。在2周时观察到华勒氏变性和轴突再生模式，所有组在8周时组织学恢复。通过测试的六种方法中的任何一种诱导的神经挤压都是相似的，并提供了一种可靠的轴突断裂模型。
