Folgueira L, Delgado R, Palenque E, Noriega A R
Department of Microbiology, Hospital Doce de Octubre, Madrid, Spain.
Neurology. 1994 Jul;44(7):1336-8. doi: 10.1212/wnl.44.7.1336.
We used a test based on the polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis DNA in 11 CSF samples from 10 HIV-seropositive patients in whom tuberculous meningitis was suspected. PCR was positive in nine samples from eight patients in whom clinical data, CSF findings, evidence of tuberculosis at an extraneural site, and response to antituberculous drug therapy supported the diagnosis, whereas cultures were positive in five of these nine samples and staining in only one. The PCR was negative in 14 CSF samples from the control group.
我们采用了一种基于聚合酶链反应（PCR）的检测方法，对10例疑似结核性脑膜炎的HIV血清阳性患者的11份脑脊液样本进行结核分枝杆菌DNA检测。在8例患者的9份样本中，PCR检测呈阳性，这些患者的临床数据、脑脊液检查结果、神经外部位的结核证据以及抗结核药物治疗反应均支持该诊断，而这9份样本中有5份培养结果呈阳性，仅1份涂片结果呈阳性。对照组的14份脑脊液样本中，PCR检测呈阴性。
