聚合酶链反应在监测结核性脑膜炎患者抗生素治疗后脑脊液中结核分枝杆菌DNA的应用。
Application of the polymerase chain reaction to monitor Mycobacterium tuberculosis DNA in the CSF of patients with tuberculous meningitis after antibiotic treatment.
作者信息
Lin J J, Harn H J
机构信息
Department of Neurology, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
出版信息
J Neurol Neurosurg Psychiatry. 1995 Aug;59(2):175-7. doi: 10.1136/jnnp.59.2.175.
Abstract
The polymerase chain reaction (PCR) was used to detect Mycobacterium tuberculosis DNA in 29 CSF specimens from seven patients with tuberculous meningitis after the start of antituberculous chemotherapy. Ten of the 13 CSF specimens taken from these patients with an initial treatment of three weeks were positive for the PCR study. By contrast, only one of the other 16 CSF specimens taken from patients treated for more than three weeks was positive. This study shows that M tuberculosis DNA can exist in the CSF of a patient with tuberculous meningitis for three weeks after treatment and that PCR can still be a sensitive method to detect M tuberculosis DNA in the CSF after the start of treatment in patients with tuberculous meningitis.
摘要
采用聚合酶链反应（PCR）检测7例结核性脑膜炎患者在抗结核化疗开始后29份脑脊液标本中的结核分枝杆菌DNA。在这些接受初始治疗3周的患者所采集的13份脑脊液标本中，有10份PCR检测呈阳性。相比之下，在接受治疗超过3周的患者所采集的另外16份脑脊液标本中，只有1份呈阳性。该研究表明，结核性脑膜炎患者脑脊液中的结核分枝杆菌DNA在治疗后3周仍可存在，且PCR在结核性脑膜炎患者治疗开始后仍可作为检测脑脊液中结核分枝杆菌DNA的敏感方法。
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